Probing the chromatin structure of ribosomal DNA using extended chromatin fibers
نویسندگان
چکیده
منابع مشابه
Organization of DNA in chromatin ( chromatin structure / intercalation / DNA - kinking )
Conformational changes in DNA that accompany drug intercalation have led us to ask if DNA first bends or "kinks" to accept an intercalative drug or dye. Kinking is made possible by altering the normal C2' endo deoxyribose sugar ring puckering in B DNA to a mixed sugarpuckering pattern of the type C3' endo (3'-5') C2' endo and partially unstacking base-pairs. A kinking scheme such as this would ...
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Squashing salivary glands of Chironomus thummi larvae, Amblystoma tigrinum erythrocytes, or Spirostromum frequently results in stretched chromatin having highly oriented DNA as determined by polarized fluorescence microscopy of acridine orange-stained preparations. The examination of such material from C. thummi in the electron microscope indicates that the individual chromatin fibers have an a...
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Transcription of the major ribosomal RNAs by Pol I (RNA polymerase I) is a key determinant of ribosome biogenesis, driving cell growth and proliferation in eukaryotes. Hundreds of copies of rRNA genes are present in each cell, and there is evidence that the cellular control of Pol I transcription involves adjustments to the number of rRNA genes actively engaged in transcription, as well as to t...
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ژورنال
عنوان ژورنال: Biopolymers and Cell
سال: 2019
ISSN: 0233-7657,1993-6842
DOI: 10.7124/bc.0009de